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July 10, 2018

5

DIY Gene Editing

DIY Gene Editing

You’ve probably heard amazing stories about research using DIY gene editing technique CRISPR. The Biohacker world is captivated by this revolutionary technology. It is easier, cheaper and more efficient than other approaches to modifying DNA. Welcome to the Biohacker CRISPR Tutorial for beginners…

What Is CRISPR?

The term CRISPR/Cas9 stands for Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9. We know, this is a mouthful. Simply put, the names imitate important features recognized during its discovery. They don’t tell us much about how it works. These terms were created before anyone understood what it was. Therefore, you will most often hear the process simply referred to as CRISPR. In this Biohacker CRISPR Tutorial you will learn the basics of this revolutionary technology.

What Is CRISPR?

What Does CRISPR Do?

Before we can understand the Biohacker CRISPR Tutorial, we need to know what CRISPR is. CRISPR is a found in bacteria. It is centered on immune resistance. Microorganisms use CRISPR to cut up the DNA of invading viruses that might kill them. Today, we have modified this molecular equipment for an entirely different purpose. We can use it to powerfully change any chosen letter(s) in an organism’s DNA code. This might sound in the realm of Science Fiction, but we are on the cusp.

We might want to correct a disease-causing mistake that was inherited into our DNA when it was replicated. Or, in some cases, we may want to augment the genetic code of crops, livestock or perhaps even people. So, do we just snip the unwanted gene out and replace it with a good one? Is CRISPR really as easy as cut-and-paste in Microsoft Word? How can Mind Audio be a lateral for home biohackers? Note: Click here to read about software that can help with this process.

What Is Mind Audio?

DIY Gene Editing

We have to remember that animals and plants are composed of millions of cells. Each cell contains the same DNA. You can’t edit just one cell, there would be little point. We would have to edit the same gene in every single cell. This would consist of millions of separate cut-and-paste operations. Also, not all cells are easy to get to. How could we reach cells buried in our bones? What about brain cells? A much better approach is to start at the beginning. You can then control the genome while there is only one cell.

The solution is to start with a very early embryo. Because of this, all we need is a giant microscope and a tiny pair of scissors. This is where Cas9 is intreoduced. Cas9 is the technical name for the virus-destroying “scissors” that progressed in microorganisms. The CRISPR part of the name comes from repeat DNA arrangements that were part of a multifaceted structure telling the scissors which part of the DNA to cut.

DIY Gene Editing Biohacker CRISPR Tutorial

Biohacker CRISPR Tutorial

Remember, DNA comes in two strands. One strand fits alongside the other. We make a guide with a code that will line up with only one part of our 3 billion base pair long genome. Then, our “scissors” can make the cut in precisely the right place. The Cas9 scissors slice the DNA just where we intend. The cell will then try to repair the break using any available DNA it can find. So, we also inject the new gene we want to insert.

DIY Gene Editing Biohackers use a microscope and a tiny needle to inject the CRISPR/Cas9 together with the guide, the donor DNA, and the new gene. How does the new gene find the right place to embed itself? First, the guide will shake along and find the right place for the scissors to cut. Then, the new contributor DNA will line up where it fits. It will be permanently stitched into the DNA strand via natural DNA repair mechanisms.

Recently, though, new CRISPR editing systems have been created that don’t even require a cut through the DNA. In this case, the CRIPSR/Cas and guide system can deliver an enzyme to a particular gene and modify it, rather than slicing anything out. Some are even experimenting with completely virtual technologies and analogs like brainwave hacking with iDoser.com, as an example.

Binaural Beats Tested

What Can You Do With CRISPR?

Some DIY Gene Editing Biohackers use mouse embryos or cells grown in petri dishes. Researchers modify stem cells to repopulate injured organs. A few laboratories around the world are working with early human embryos. This research is highly controlled and judiciously watched. Some are working with virtual audio technology that uses brainwaves as a simulated biohacking lateral. Companies like iDoser.com are leading this space. Others work on plant cells. Just a few cells grow entire plants. Think of how revolutionary this might be for the Marijuana, Corn, Hemp, Cotton and many other industries.

As we learn more, the scope of what we can do with CRISPR/Cas9 will advance. Every organism and every cell is different. What’s more, everything in the body is connected, so we must think about unforeseen side effects. We must consider the ethics of changing genes. Most of all we, as a society, should discuss and agree what we wish to accomplish with this revolutionary technology.

5 Comments Post a comment
  1. Tea Larsen
    Jul 10 2018

    I have seen these CRISPR kits online. I think many of them are just scam artists selling some generic lab equipment. Correct me if I am wrong? I will leave these to the lab coats. I do like the idea of a little brainwave modification with idoser and a virtual. I assume it’s not actually gene editing, though?

    Reply
  2. Franklyn Waters
    Jul 10 2018

    I’m really scared of what will happen if this becomes too easy. It should be very highly watched and regulated. I’m usually all for hacking, but not this.

    Reply
  3. Miah Barnes
    Jul 10 2018

    Correct, a lot of us biohackers are using tools like i-doser to run alongside various other hacking methods. I believe the point here was that binaural tech is lateral. Use CRISPR kits for dna, binaural stuff from i-doser for the brain, or even as an enhancement. Don’t freak out. Most of us biohackers are just hacking plants (I hope). I grow using a combination of biohacking and binaural audio from i-doser and I have never seen bigger yields, ever.

    Reply
  4. Claude Castillo
    Jul 10 2018

    No to human or animal cloning. I just read there are companies offing to clone pets? Is that true? Do you get an exact reproduction of your dog? Anybody know?

    Reply
  5. Xavier Vickers
    Jul 10 2018

    I think this is getting a little blown out of proportion. A CRISPR kit is just some specialized lab equipment. Nobody is cloning people or cats out of their garage. Not a single person as biohacked a super-human ability via gene editing. Many of us use plants to experiment with, not rats.

    Reply

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